Biodegradation of Carcinogenic Acrylonitrile Residues Using Microorganisms

Abstract

: A series of Acrylonitrile (CR
3RHR
3RN) concentrations were prepared within the purity of 96%,
which were (50, 100, 150, 200, 500 and 1000 ppm), A pure culture of Bacillus subtilis bacteria was
obtained to determine its efficiency in Acrylonitrile biodegradation, the bacterial culture was concentrated
using enrichment media (Brain Heart Infusion – BHI). The Acrylonitrile concentrations under study were
inoculated with 1.5×10P
8
P cell/ml of bacterial culture inference by McFarland turbidity standards (600 nm,
OD=0.5), and were incubated for 7 days at 37° C, then the concentrations were measured before and after
the biological treatment using HPLC in order to determine the efficiency of the bacteria in the
biodegradation process. Results showed that the optimum concentration for treatment is 500 ppm with a
percentage of 95.26%